Expression of proto-oncogenes in afatoxin B1-induced rat hepatocellular carcinomas and cultured cell line, Kagura-1

F. TASHIRO; S. MORIMURA; K. HAYASHI; R. MAKINO; H. KAWAMURA; N. HORIKOSHI; K. NEMOTO; K. OHTSUBO; T. SUGIMURA; Y. UENO

doi:10.2520/myco1975.1986.29

Expression of proto-oncogenes in afatoxin B₁-induced rat hepatocellular carcinomas and cultured cell line, Kagura-1

F. TASHIRO, S. MORIMURA, K. HAYASHI, R. MAKINO, H. KAWAMURA, N. HORIKOSHI, K. NEMOTO, K. OHTSUBO, T. SUGIMURA, Y. UENO

Author information

F. TASHIRO
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
S. MORIMURA
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
K. HAYASHI
Biochemistry Division, National Cancer Center Research Institute
R. MAKINO
Biochemistry Division, National Cancer Center Research Institute
H. KAWAMURA
Biochemistry Division, National Cancer Center Research Institute
N. HORIKOSHI
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
K. NEMOTO
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
K. OHTSUBO
Department of Clinical Phathology, Tokyo Metropolitan Institute of Gerontology
T. SUGIMURA
Biochemistry Division, National Cancer Center Research Institute
Y. UENO
Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science

JOURNAL FREE ACCESS

1986 Volume 1986 Issue 23 Pages 29-33

DOI https://doi.org/10.2520/myco1975.1986.29

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Abstract

In vitro culture cell line, Kagura-1, was established from a rat hepatocellular carcinoma induced by aflatoxin B₁ (AFB₁). When the cells at passage 15 were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal calf serum in collagencoated dishes, the population doubling time and saturation density were 13.2 hr and 1.9×10⁵ cells/cm², respectively. The established cells formed colonies in soft agar.We examined the expression of proto-oncogenes in two liver tumors induced by AFBI and Kagura-1 cell. Both c-myc and c-Ha-ras expressions were activated at high levels in both tumors and Kagura-1 cells.

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