Detection of aflatoxin B1 by direct ELISA

Y. ITOH; E. HIFUMI; K. SUDOH; T. UDA; K. OHTANI; O. KAWAMURA; S. NAGAYAMA; S. SATOH; Y. UENO

doi:10.2520/myco1975.1987.26_31

Detection of aflatoxin B₁ by direct ELISA

Y. ITOH, E. HIFUMI, K. SUDOH, T. UDA, K. OHTANI, O. KAWAMURA, S. NAGAYAMA, S. SATOH, Y. UENO

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JOURNAL FREE ACCESS

1987 Volume 1987 Issue 26 Pages 31-35

DOI https://doi.org/10.2520/myco1975.1987.26_31

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Abstract

A direct enzyme-linked immunosorbent assay (ELISA) for the quantitation of aflatoxin B₁ (AFB₁) was developed by the utilization of horseradish peroxidase (HRP) labelled anti-AFB₁ monoclonal antibody and 96-well immunomicroplate coated with AFB₁-bovine serum albumin conjugate. The detection limit for AFB₁ was ca. 5 pg/assay. A quick ELISA system was also developed using the 8-well immunomicroplate, and ca. 50 pg to 5 ng/assay of AFB₁ were quantitated within 10 min.

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