A direct enzyme-linked immunosorbent assay (ELISA) for the quantitation of aflatoxin B
1 (AFB
1) was developed by the utilization of horseradish peroxidase (HRP) labelled anti-AFB
1 monoclonal antibody and 96-well immunomicroplate coated with AFB
1-bovine serum albumin conjugate. The detection limit for AFB
1 was ca. 5 pg/assay. A quick ELISA system was also developed using the 8-well immunomicroplate, and ca. 50 pg to 5 ng/assay of AFB
1 were quantitated within 10 min.
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