A Survey for the aflatoxins in secondary metabolites produced by Aspergillus orgzae and Aspergillus sojae by enzyme-linked immunosorbent assay and high performance liquid chromatography

Abstract

The production of aflatoxins (AFs) by Aspergillus parasiticus NRRL 2999 attained maximum 144 hr after inoculation and the incubation time was used for subsequent experiment. AF producibility of 34 strains belonging to A . oryzae and A. sojae were inspected by enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC). All 34 strains did not produce AFs. On the other hand, the presence of AFs-like material was confirmed by HPLC, and its retention time was similar to but different from that of AF B₂. The AFs-like material was not detected by ELISA.