1998 Volume 1998 Issue 47 Pages 33-38
Emodin (EM) is an anthoraquinoid mycotoxin and a component of medicinal plants, and 2-hydroxy-emodin (2-OH-EM) is one of the active metabolites of EM. Effects of 2-OH-EM and EM on the activation of 2-amino-3-methyl-imidazo [4, 5-f] quinoline (IQ) was evaluated using a Salmonella - microsome system (Ames test) in the presence of 7, 8-benzoflavone (BF), an inhibitor of hepatic cytochrome P-4501A. Using the Ames test with the Salmonella Typhimur-ium strain TA98 (TA98), 2-OH-EM and EM almost completely inhibited the mutagenicity of IQ (4 ng/plate) at 5μg and 1μg/plate, with IC50 values of 3.0 and 0.7μg/plate, respectively. With the S. Typhimurium strain TA1537 (TA1537), no decrease of IQ-induced revertant numbers was observed for either 2-OH-EM or EM. While IQ showed no effect on the mutagenicity of 2-OH-EM, it reduced the mutagenic activity of EM by 50%. These data suggest that the anti-mutagenic effect of EM and its metabolite can be attributed to the competitive inhibition of the microsomal activation of IQ. The present study showed for the first time the antimutagenic activity of 2-OH-EM against cooked-food mutagens in TA98 and TA1537 with S9 mix.
