Mycotoxin Analysis Using Immunoaffinity Columns

Abstract

To minimize the interference of co-extracted compounds from foods and feeds in which mycotoxins occur, the conventional methods for chemical analysis of mycotoxins consume large amount of time and solvent, need several cleanup steps, and sometimes require practi-cal experiences. From the environmental point of view, toxic solvents such as chloroform and benzene used for mycotoxin analysis should be saved from now on. The immunoaffinity column (IAC) using anti-mycotoxins antibodies are highly specific, simple and rapid, and saving toxic solvents. Moreover, recently, with the availability of commercial IACs for aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEN), fumonisins and deoxynivalenol (DON), these IACs have become the powerful tools in cleanup stage of mycotoxin analysis. This paper describes the performances of IACs in mycotoxin analysis, reuse of IAC, and multimycotoxin analysis using linked different IACs.