Abstract
In this study we studied the contamination of AFB1 in corn seeds (N=28) and ground peanut (N=28) in northern Thailand. The samples were extracted with chloroform for TLC and with 80% methanol for competitive enzyme-linked immunosorbent assay (cELISA). The levels of AFB1 in corn seeds determined by TLC and ELISA method were ranged from 0 to 540 ppb and 0 to 576 ppb; and in ground peanut from 0 to 695 ppb and 4 to 450 ppb, respectively. The minimum detectable limit of the ELISA with the most sensitive monoclonal antibody (MAb) AF5 was 1 ppb while that of TLC was 10 ppb. The correlation coefficients between our own ELISA with commercial ELISA kits, and TLC method were 0.92 and 0.87 for corn seeds (p<0.05), and 0.94 and 0.84 for ground peanut (p<0.05), respectively.
