Abstract
Rapid strip test kits (AgraStrip) were validated to test total aflatoxins in corn using ethanol extraction method at cut-off levels of 4 μg/kg, 10 μg/kg and 20 μg/kg, respectively. The test is a one-step lateral flow immunochromatographic test based on a competitive immunoassay format. The strip membrane contains a test zone and a control zone. A positive sample with total aflatoxins greater than the cut-off level will result in no visual line in the test zone. Alternatively, a negative sample with total aflatoxins less than the cut-off level will form a visible line in the test zone. One line will always be visible in the control zone regardless of the presence of aflatoxins. The test is a rapid semi-quantitative method with assay results to be obtained within 5 minutes. Validation studies assessed the accuracy of test kits by using ethanol as sample extraction solvent. Results indicated that the tests are accurate and effective for semi-quantitive measuring total aflatoxins greater or less than the cutoff levels in corn.
