Production of polyclonal antibody against fumonisin B1 and detction by ELISA in Thailand

Abstract

Polyclonal antibodies against fumonisin B 1 (FB1) were produced in the rabbits after immunizing the animal with FmBI-brovine serum albumin. A direct competitive enzyme-link immunosorbent assay (ELISA) was used to characterize the antibodies and for analysis of the toxin in corn samples. The serum against FB1 was obtained from the rabbit after 5 weeks of immunization. The highest antibody titer was found in the sera of rabbit 10 weeks after immunization with the titer of 1:25, 000 The conjugation of FB 1-HRP was achieved by a water soluble carbodiimide (EDPC). The appropriate time of the reaction between FB 1 and FB 1-HRP in competition to bind the antibody at the bottom of the well was 60 minutes. A good correlation was found between FB 1 level in naturally contaminated corn samples analyzed by direct ELISA and the high pressure liquid chromatography (HPLC) method. The correlation coefficient of a linear regression between ELISA and HPLC data was found to be 0.9732.