FAPAS proficiency testing of aflatoxin analysis in food and feed using DOA-Aflatoxin ELISA test kit

Abstract

DOA-Aflatoxin ELISA Test Kit is an in-house (ELISA test) kit produced by Department of Agriculture (DOA) Ministry of Agriculture and Cooperatives. The test kit had been validated for their precision and accuracy for aflatoxin B₁ detection in agricultural commodities. However, proficiency testing of analysis using DOA-Aflatoxin ELISA test kit is an essential element of test kit quality assurance. The analysis performance could be compared the analytical results with those from other laboratories with different methods. The test materials of animal feed, pistachio paste, peanut butter slurry, peanut and chilli powder were prepared and sent to our laboratory by FAPAS (Food Analysis Performance Assessment Scheme). The analysis was performed for aflatoxin B₁ using DOA-Aflatoxin ELISA test kit. The satisfactory results of Zscore were obtained from the tests. Performance in a FAPAS proficiency testing, therefore is considered satisfactory if a participant's Z-score lies within the range of-2 and +2.