Application of one-step reverse transcription loop mediated isothermal amplification (reverse transcription LAMP) for rapid detection of fungal gene expression in pure culture mycelia and in planta

Ekaterina Minenko; Rudi F. Vogel; Ludwig Niessen

doi:10.1016/j.myc.2014.01.009

Note

Application of one-step reverse transcription loop mediated isothermal amplification (reverse transcription LAMP) for rapid detection of fungal gene expression in pure culture mycelia and in planta

Ekaterina Minenko, Rudi F. Vogel, Ludwig Niessen

著者情報

キーワード: Ef1α, Infection, mRNA, Plant, β-Tubulin

ジャーナルオープンアクセス

2014 年 55 巻 6 号 p. 425-430

DOI https://doi.org/10.1016/j.myc.2014.01.009

詳細

抄録

A novel application for reverse transcription loop-mediated isothermal amplification (reverse transcription LAMP) was developed which enables rapid, accurate and inexpensive detection of gene expression in pure culture mycelia with no previous mRNA isolation necessary as well as in infected plant tissue after mRNA isolation. Usefulness of the method was demonstrated by detection of mRNA templates of the Fusarium graminearum housekeeping genes coding for Ef1α and β-tubulin, in mycelia and in planta.

責任著者(Corresponding author)

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