MICROSCOPICAL OBSERVATION ON THE LIVER OF THE LIVING MICE TREATED WITH VARIOUS MEDICINES
I. ON THE NORMAL CASE AND THE CASE OF INJECTED DYE
1957 Volume 45 Issue 11 Pages 1234-1241
Details
1957 Volume 45 Issue 11 Pages 1234-1241
1. The intrahepatic circulation of the blood in urethan-anesthetized mice in the normal case or the case of injected dye, has been studied by means of the transillumination technique with the long length condensor type apparatus.
2. In some sinusoids is noticed a pause of circulation and loss of the corpuscles after slowing down for a while; in some sinusoids, the corpuscles remain stagnant in them throughout a period of inactivity, and at the same time, in some sinisoids the corpuscles circulate rapidly. In this case, the blood-flow in sinusoids is variable and such variation, as above mentioned, is not caused by diffinitive rhythms.
3. In the intrahepatic circulation, irregular pause is observed in some portion of the lobule. No evidence, however, that over 75% of capillary circulation may be stopped in normal circulation; also in intralobular circulation it is recognized no finding that the flow and the pause may be repeated on every lobule alternately.
4. No regulatory mechanism of the circulation is observed in the beginning or the end of the sinusoids. Narrow portions, however, that seem to be the sphincteric muscle, are noticed in termination of some sinusoids in the lobules, whereas in fact there is no such mechanism as the sphincteric muscle.
5. On peripheral observation, 3 connecting ways are recognized in tying up the portal twig with the central vein.
6. No blood-flow of hepatic aretrioles is recognized with above mentioned procedure.
7. After injection of India ink or Sumi, the Kupffer's cells, as well as the facets of liver cells, are noticed more apparently and the difference is recognized in degree of their phagocytic activity. To find the bile ductule in Glisson's sheath and Disse's space with this technique is not accomplished yet.
