STUDIES ON THE LEUCOCYTE-INCREASING-SUBSTANCES

(6) INFLUENCE OF CYSTEINE ON THE BONE MARROW PICTURE OF THE RABBIT

Abstract

What is the relation between the leucocytosis in the peripheral blood caused by the intravenous injection of cysteine and the bone marrow cell? In order to make researches upon this problem, 6mg of cysteine was given intravenously to the normal rabbits and the bone marrow pictures were studied 4 hours and 8 hours after the injection-when the increase was observed in the leucocyte count in the peripheral blood-and also after 24 hours-when the count dropped back. The results are as follows: After 4 hours and 8 hours the nucleated cell count decreased markedly with the variations of the bone morrow picture primarily due to pseudoeosinophils, and especially mature cells diminished. while, the immature pseudoeosinophils were observed to have a tendency to increase to some extent. Considering the above results, the leucocytosis with characteristics two-peaks-mainly due to pseudoeosinophil increase by cysteine administration-is not resulted from the release of the reserved leucocytes from the blood cell reservoirs, but obviously from a fact that the leucocyts is mobilized out of the bone marrow. Further, the previous reports and literature lead us to infer that the first peak of leucocytosis, appearing 4 hours after the injection of cysteine, is formed by the direct action of cysteine itself to the bone marrow, and that the second peak occurring 8 hours after the injection is produced by secondarily produced Neutrophilin (Neutropoetin) acting to the bone marrow-both cysteine and neutrophilin (Neutropoetin) promotes hematopoiesis of pseudoeosinophils while inducing the mature pseudoeosinophils to be mobilized and to move out.