MICROSCOPIC OBSERVATIONS OF THE LIVER UNDER ACCELERATED HEPATIC CONDITIONS IN THE LIVING ANIMAL

II. IN VIVO OBSERVATIONS WITH TRANSILLUMINATION TECHNIQUE IN NEPHRECTOMIZED ANIMALS

Abstract

Observations were made with “transillumination technique” of the liver in situ in rats and mice in which both kidneys had been removed. The following are the conclusions:
1) Shortly after bilateral nephrectomy, sinusoids are somewhat dilated, the velocity of blood stream slightly accelerated, but no demonstrable changes occur in the liver cells, biliary capillaries and Kupffer's cells. If a dye, azorbin S, was infused, appearance and disappearance of the dye granules were faster in nephrectomized animals than normals. The effects of the injection of hepatotonica (Ca-or Na paraoxybenzoate, Na hippurate, ACTH) or cholagoga (Na dehydrocholate) upon the blood stream, sinusoids and movement of dye were greater in such mice than normals.
2) At 24 hours after bilateral nephrectomy, the blood vessels are considerably dilated, and both velocity of blood stream and blood volume increased. These changes are more marked than shortly after nephrectomy. The influence of hepatotonica upon such changes and the excretion of the dye into the bile are also greater at 24 hours.
3) The observations made one week after unilateral nephrectomy were similar to those made immediately after bilateral nephrectomy.