Fibronectin Immobilization onto Titanium using Tresyl Chloride-activation Technique

抄録

Tresyl chloride activation technique was new method for immobilizing proteins onto titanium surface. In the present study, fibronecitn was immobilized onto titanium surface using tresyl chloride-activation technique. Titanium surface was mirror polished, or pretreated with H₂O₂/NaOH aqueous solution for 30 min or 90 min after mirror polishing. Each titanium surface was covered with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) and was stored at 37°C for 2 days. The basic terminal OH groups of titanium were reacted with tresyl chloride. The tresylated titanium disk was then immersed into a fibronectin/PBS solution. H₂O₂/NaOH pretreated titanium surfaces had less degree of tresylated OH groups compared with mirror polished titanium, but the intensity of N1s peas which was derived from immobilized fibronectin was almost the same between mirror polished and H₂O₂/NaOH pretreated titanium. Finally, fibronectin could be easily immobilized onto each titanium surface using tresyl chloride-activation technique.