Interleukin-6 Expression in Three-dimensional Tumor Microenvironment                    using HSC-4 Cells

Tamaki NAKANISHI; Kaname TSUJI; Yuki MATSUSHIMA; Koji YAMADA; Kazuhiro MATSUMOTO; Hiroaki YOSHIDA; Tomio ISEKI; Hideo SHIMIZU; Shosuke MORITA

doi:10.11344/nano.10.21

ORIGINAL ARTICLES

Interleukin-6 Expression in Three-dimensional Tumor Microenvironment using HSC-4 Cells

Tamaki NAKANISHI, Kaname TSUJI, Yuki MATSUSHIMA, Koji YAMADA, Kazuhiro MATSUMOTO, Hiroaki YOSHIDA, Tomio ISEKI, Hideo SHIMIZU, Shosuke MORITA

Author information

Tamaki NAKANISHI
Graduate School of Dentistry (Oral and Maxillofacial Surgery),
Kaname TSUJI
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Yuki MATSUSHIMA
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Koji YAMADA
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Kazuhiro MATSUMOTO
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Hiroaki YOSHIDA
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Tomio ISEKI
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Hideo SHIMIZU
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,
Department of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Osaka, Japan
Shosuke MORITA
First Department of Oral and Maxillofacial Surgery, Osaka Dental University, Osaka, Japan,

Keywords: spheroid, IL-6, HSC-4 cells

JOURNAL FREE ACCESS

2018 Volume 10 Issue 1 Pages 21-25

DOI https://doi.org/10.11344/nano.10.21

Details

Abstract

Reproduction of tumor microenvironment in vivo using the conventional 2- or 3-dimensional culture method is difficult because of the lack of extracellular matrix (ECM) and intercellular interaction. In this study, we tried to establish a 3-dimensional tumor environment using spheroids of oral cancer-derived cells. The Interleukin-6 (IL-6) expression level was high in the spheroids on measurement using the real-time RT-PCR method showing a difference from that in single cells. A representative inflammatory cytokine, IL-6, increases the activity of many types of cancer-derived cells, being involved in tumor formation and metastasis, and similar findings were noted in HSC-4 cells derived from human squamous cell carcinoma of the tongue, for which analysis of phenomena in the cellular interface in 3-dimensionally formed masses is expected. It is highly possible that this method is applicable for studies on the carcinogenicity of nanomaterials represented by asbestos.

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