Cell Viability of KF and NaF using Cell Recovery Tests between Two- and Three-dimensional Cultures using Mouse-derived ES-D3 and 3T3 Cells

Abstract

An in vitro test to assess the toxicity levels of chemicals based on the recovery of cell growth in a normal culture environment after transient exposure is used as a cell recovery test. This test is generally conducted in a two-dimensional culture. As human tissues and organs have a complicated three-dimensional structure, cell recovery tests developed in three-dimensional tissue models may be superior to those in two-dimensional cultures.

Fluorides are commonly employed in the dental field to prevent dental caries. However, their effects on human health are attracting social attention. In the present study, KF and NaF were evaluated by comparing the results of cell recovery tests between two- and three-dimensional cultures using mouse-derived ES-D3 and 3T3 cells. As a result, ES-D3 cells exhibited more significant recovery than 3T3 cells after exposure to both chemicals in the three-dimensional culture compared with in the two-dimensional culture.