Cell Viability of Fibroblast Growth Factor 2 (FGF2) under Serum Addition Conditions using Mouse-derived Cells

Abstract

Cells derived from animals other than humans are used in the cytotoxicity screening tests of many pharmaceuticals and quasi-drugs used in humans. FIBLAST^® spray contains recombinant human FGF2 protein as a main ingredient for which data concerning the proliferation of various cells are available. We investigated the influence of this product on the cell viability of mouse-derived Balb/c 3T3 cells. Under the condition of the addition of FIBLAST^® solution, unlike human-derived cells, the viability decreased in about half of the cells, clarifying the cytotoxicity of the product, and this was not dependent on the serum concentration of the culture medium. It was suggested that a species difference between humans and animals influenced the result. However, the influence of additives is also considered because many additives are mixed in FIBLAST^® spray, so it was not possible to conclude that the test result was due to the influence of a species difference. It may be necessary to compare the result with the influence of trafermin alone in the future.