Surface Pre-reacted Glass-ionomer (S-PRG) Filler Eluate Suppresses MMP-1 Secretion by TNF-α-stimulated Human Gingival Fibroblasts

Abstract

Tumor necrosis factor (TNF)-α is a major inflammatory factor that regulates tissue destruction in periodontitis and promotes the release of Matrix metalloproteinase (MMP) -1, which breaks down the gingival extracellular matrix. MMP-1 is one of the proteolytic enzymes that are capable of cleaving collagen and may play an important role in the destruction of connective tissue. The present study investigated the effects of Surface pre-reacted glass-ionomer (S-PRG) filler eluate on the secretion of MMP-1 by human gingival fibroblast (HGF) stimulated with the inflammatory cytokine TNF-α. S-PRG filler eluate did not alter Akt or nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 phosphorylation levels in TNF-α-stimulated HGF. In the mitogen-activated protein kinase (MAPK) pathway, S-PRG filler eluate did not alter the phosphorylation level of p38 in TNF-α-stimulated HGF, but decreased that of c-Jun N-terminal kinase (JNK).

These results will facilitate the development of new anti-inflammatory agents for periodontal disease using materials composed of S-PRG filler eluate.