2022 Volume 14 Issue 2 Pages 61-66
In a phototoxicity test, a solar simulator with a tungsten lamp, of which the calorific power is great, is used, but, concerning the LED-type irradiator that we developed, the influence of heat on the cultured cells can be mostly ignored, and an experiment may be conducted while placing the LED closer to the cultured cells. No study has examined the distance between cultured cells and a light source. In this study, we established the distance between cultured cells and a LED: short and long distance groups, and investigated the 3T3 cell viability using an MTT assay to clarify the influence on the phototoxicity level in the Bis-GMA + Camphorquinone (CQ) and Bis-GMA groups.
The results showed that cell viability rapidly increased with an increase in the dilution ratio in the long distance group. However, in the short distance group, it only slightly increased with an increase in the dilution ratio. In the Bis-GMA group, photoirradiation slightly influenced cell viability. There was a marked change in phototoxicity related to the distance between the LED and cells. As light-cured composite resin contains a large amount of nanofiller, the influence of composition with a nanomaterial should be examined in the future.
