Sealing Property of Dentin Tubules by A New Hypersensitivity Control Material and Its Effect on Human Cultured Cells

Abstract

This study was conducted to observe the dentin tubule sealing and biocompatibility of a newly developed dental hypersensitivity control material. The Fourth Dentin^® (4D) made mainly from nano-sized calcium carbonate was applied to bovine anterior dentin and observed vertically using a scanning electron microscope. Because 4D forms film on dentin and 4D vertically seals dentin tubules, human periodontal ligament fibroblasts, human deciduous dental pulp-derived fibroblasts, and human osteoblasts were cultured with medium-added 4D. As a result, the cell proliferative ability of each cell to which 4D was added was enhanced. Each cell was also reacted with 4D and the condition of the cells around 4D was observed. The results showed that 4D enhanced mitochondrial activity in human periodontal ligament fibroblasts, human deciduous dental pulp fibroblasts, and human osteoblasts, and that each of these cells proliferated around 4D, suggesting a biocompatibility between these cells and 4D.