2024 Volume 16 Issue 1 Pages 51-58
Lipopolysaccharide (LPS) is a potential inducer of chronic inflammation, cellular senescence, and impaired bone regeneration. LPS sustained-release gelatin (LS-G), developed in our previous studies, is a useful chronic inflammation-inducing device; the application of LS-G has the potential to freely induce and regulate inflammation and cellular senescence in animal models via the sustained release of LPS and may be useful in elucidating pathological mechanisms. However, there is a paucity of information on the effects of conditions such as LPS concentration and the number of dehydrothermal treatments (DHT) on LS-G. Here, we investigated the effect of adjusting the number of DHT (1vh, 2vh) and concentration of LPS (×1, ×6, ×10, ×100) on the LS-G properties. First, we showed that implantation of LS-G with high LPS concentration can induce chronic inflammation in vivo. Next, we compared the LS-Gs under each condition for the properties, such as the micro-structure, water absorption time, and the LPS release behavior. The microstructure of the LS-G surface showed a few changes depending on the concentration of LPS or the number of DHT. The water absorption time significantly increased with the number of DHT. The LPS concentration was found to be related to the release of LPS. These results suggest that regulation of the number of DHT and the concentration of LPS may be useful for modulating the properties of LS-G. This study may lead to the development of highly functionalized LS-G that contribute to the development of an efficient model of chronic inflammation, cellular senescence, and impaired bone regeneration.
