Abstract
We examined the differentiation capacity of mouse embryonic stem (ES) cells cultured on C60 fullerene, as well as cell viability effects, employing a cellular differentiation technique for the embryonic stem cell test (EST). The results demonstrated minor effects on differentiation assay, beating myocardial rate of the dispersed solution was noted in 72% of the control. In addition, there was no influence on cell viability. In this study, the C60 fullerene did not influence the differentiation of ES-D3 cells and "non embryotoxicity". We here investigated C60 fullerene dispersed in a medium. We expected a result of natural dispersion of C60 fullerene into the medium by agitation as possible. This is probably because C60 fullerene did not dispersed. Fullerene particles should be sufficiently dispersed in media. In the future, the biological safety should be comprehensively ex-amined by improving dispersion in medium.
