Abstract
Why do mesenchymal stem cells (MSCs) regenerate good cartilage tissue in vitro under condition of microgravity generated by a rotating vessel? To answer this question, we developed a PIV measuring apparatus equipped with a RWV (rotating wall vessel) bioreactor, which enabled visualization of the three dimensional medium flow applied to the aggregated cartilaginous cells. The spacial distribution of cartilage marker genes (sox-9, aggrecan, and collagen type II) was measured using a microdisection system and discussed in association with the PIV results.
