Abstract
Authers investigated the chromosomal aberrations of sixteen astrocytomas in fresh specimens and paraffin blocks by fluorescent in situ hybridization (FISH) and tried to use FISH to investigate paraffin blocks for retrospective study in astrocytomas. Each fresh speciman was divided into two parts. The part of tissue was made in single cell suspension and hybridized with biotined centromeric DNA probes D7Z1, D9Z1, D10Z1 and D17Z1. The other one was embedded with paraffin routinely and made in single cell suspesion. Before hybridization, pretreatment of cells with proteinase K was carried out. Hybridization spots were counted under fluorescence microscope. The results showed that the number of hybridization spots of fresh specimen and paraffin block in a sample was almost same for all the probes. There were eleven cases with trisomy 7, eight cases with monosomy 10, three cases with tetrasomy 17 and two cases with monosomy 9 in this group. Our result suggests: (1)FISH is advantage and simple to analyze chromosome aberration in human astrocytomas. (2)The aberrations of chromosome 7,9,10 and 17 are common in human astrocytomas, including trisomy 7 monsomy 10, tetrasomy 17. (3)The combination of trisomy 7 and monsomy 10 is probly involved with progression of glioblastoma. (4)FISH method can be applied to observe chromosomal numeric changes in paraffin block for retrospbctive studies.
