中枢神経系に対する低周波超音波照射とEdaravone

抄録

Purpose: The thrombus dissolution efficiency of tPA is enhanced by ultrasonic irradiation. Furthermore, a free radical scavenger “Edaravone”is used frequently in Japan. Considering the clinical situation, the safety of applying ultrasonic irradiation at the time of Edaravone medication was evaluated. Methods: Organ-cultured slices of mouse brain and human neuroblastoma SK-N-MC cells were used. Four groups were set up: a control group, an Edaravone medication group, an ultrasonic irradiation group, and a group in which both methods were combined. Six replicates were used for evaluation with mouse brain slices and 2 for evaluation with SK-N-MC cells. Ultrasonic irradiation was applied at 500kHz and 0.3W/cm2 with a continuous sine wave, with intermittent application. Edaravone was used at 6μM. Cell death in the mouse brain slices was evaluated by PI staining over 28days. The number of apoptotic SK-N-MC cells was evaluated by the TUNEL and FACS methods. Results: There was no increase of dead cells in the mouse brain slices, or of apoptotic SK-N-MC cells, in any of the groups. Conclusion: Our findings suggest that ultrasound and Edaravone do not injure cells in the central nervous system.