Allozyme variation of Nuphar subintegerrimum (Nymphaeaceae) at Kita-Nagaike pond, Kakogawa City, Hyogo Prefecture, Japan.

Abstract

A natural populatio nof Nuphar subintegerrimum in an irrigatio npond was threatened by highway construction and its transplantation was planned. Allozyme variations of 60 plants belonging to three separate patches were examined in order to make a proper design assessment of transplantation including the conservation of genetic diversity of the population. Based on the variations of Pgi-2 and Tpi-3, a total of four genotypes were recognized. The distribution patterns of each genotype indicated the dominance of vegetative reproduction by creeping rhizomes. However occurrence of different genotypes in one population suggested occasional establishment of seedlings produced sexually. Duplicated isozyme number of PGI and TPI suggested that N. subintegerrimum is genetically polyploid.