薄層板上の酵素反応を利用する極微量リン脂質の分離定量
1990 年 1990 巻 8 号 p. 896-897
詳細
1990 年 1990 巻 8 号 p. 896-897
The purpose of the present study was to conduct a basic work of the tr ace analysis of phospholipids in biological urine and serum samples. Phospholipids were extracted with chloroform using Extrelut column and developed by TLC. Solutions of PL-C and Al-P were sprayed on the TLC plate (silica gel), and released phosphate was detected with molybdate-Malachite Green solution. This method can be easily used for the separation and determination of phospholipid content as low as pmol.
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