1991 年 1991 巻 12 号 p. 1688-1690
The regeneration of nicotinamide-adenine dinucleotide (reduced form NADH) by the reduction of NAD+ with hydrogen gas was carried out in the presence of the hydrogenase from Alcaligenes eutrophus, and the synthesis of malic acid was attempted by a combination of the above system and malate dehydrogenase. After 2 h, the highest conversion of oxalacetic acid and turnover number of NAD+ were 64% and 13, respectively.
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