Abstract
Fibrous adsorbent (Cell-PEI) for selective removal of endotoxin was prepared by immobilization of polyethyleneimine onto cellulose fiber. The anion exchange capacity of the Cell-PEI was easily adjusted by changing the ratio of polyethyleneimine to the cellulose. The endotoxin-adsorbing capacity of the adsorbent increased with increase in anion exchange capacity of the adsorbent, but decreased with increase in ionic strength of the buffer. The adsorbent (anion exchange capacity 1.1 3.0 meq/g), however, retained a high endotoxin-adsorbing activity at a wide range of ionic strength (μ= 0.05-0.4). The adsorbent (anion exchange capacity 1.1 meq/g), furthermore, selectively removed endotoxin in a bovine serum albumin (BSA) solution at an ionic strength of μ=0.05-0.2 and at around neutral pH, without affecting the recovery of the BSA.
