Influence of Solubilized Collagen Concentration on Hydrolysis of α-Tricalcium Bis(phosphate)

Abstract

The α -tricalcium bis (phosphate) (α -TCP) was hydrolyzed in solubilized collagen solutions of several collagen concentrations (0.05 to 0.4%) for pH 5 at 30° C for 24 hours. The resulting hydrolyzed products were investigated by X-ray diffraction, Fourier transform infrared spectroscopy, thermal analysis and chemical analysis. The main hydro lyzed products were hydroxyapatite (HAp) and octacalcium dihydrogen hexakis (phosphate) (OCP). The formation of HAp and OCP was accelerated in solutions of collagen concen trations over 0.2%. It was suggested that collagen was dispersed uniformly in hydrolyzed products and formed composite with HAp and OCP by interaction among them. HAp in the hydrolyzed product was nonstoichiometric hyd roxyapatite (DAp). As the product contained OCP and collagen in addition to DAp, it might be useful as a raw material of bio-materials.