Abstract
This experiment was designed to product intrahepatic gllstones by stasis of bile flow in dogs. After cholecystectomy a vinyl tube was inserted through the cystic duct into the hepatic duct of one of the middle lobes, thus causing a partial obstruction of the duct.
(1) After one year and three months intrahepatic gallstone formation was observed in the intrahepatic biliary tree and that hepatic duct.
(2) Chemical and infrared spectrum analysis showed that the stones were composed mainly of bilirubin, over 54 per cent, quite similar to human intrahepatic gallstone.
(3) Histological findings of the liver.
(i) The middle lobe with dark brown stones showed periportal fibrosis, infiltration of lymphocytes around Glisson's sheath and dark brown granular biliary sands in the relatively large intrahepatic ducts.
(ii) Other hepatic lobes showed no marked change.
(4) It will be expected that intrahepatic gallstone formation by stasis needs a long duration over one year.
