The ³⁵S Uptake of the Gastric Wall and its Excretion into the Gastric Juice in Rats with Experimental Ulcer

Clamping and Clamping-Cortisone Ulcer

Abstract

The changes of the³⁵S uptake of the gastric wall and of the ³⁵S excretion into the gastric juice were studied by using the experimental ulcer which was induced by clamping and clamping-cortisone methods in rats.
Furthermore, the effects of different steroidal hormones and non-steroidal anti-inflammatory drugs on the ³⁵S uptake of the gastric wall with ulcer were also studied.
The results of these studies are summarized as follows:
1) The ³⁵S uptake of the gastric wall and the ³⁵S excretion rate into the gastric juice increased remarkably, in the stomach with ulcer induced by the clamping method (C ulcer) compared with that of the normal stomach. The ³⁵S uptake of the ulcerated portion was especially high.
2) The ³⁵S uptake of the gastric wall increased as progress of ulcer continued and reached a maximum at the 10th ulcer day and thereafter decreased gradually in the C ulcer. These changes had a lineal relationship with an aggravating and healing condition of the ulcer.
3) In the group in which the experimental ulcer was induced by the clamping-cortisone method (C-C ulcer), the ³⁵S uptake of the gastric wall was inhibited remarkably at the 10th ulcer day. Thereafter it increased gradually as progress of the ulcer continued and reached a maximum at the 35th ulcer day. It was obvious that the ³⁵S uptake of the gastric wall in this group did not increase so rapidly compared with that in the ulcer.
A lineal relationship was observed between the degree of the ³⁵S uptake of the gastric wall and the rate of healing which was measured by histological examination.
4) At the 7th ulcer day, the inhibiting degree of the ³⁵S uptake of the gastric wall was compared in some groups treated with diff erent kinds of glucocorticoid preparations.
In two groups treated with cortisone acetate and prednisolone acetate, a clear dose-response curve was observed. However, in the groups treated with phosphate compounds, such a clear dose-response was not found. The group treated orally with prednisolone powder showed the same effect as the group treated intramuscularly.
5) The ³⁵S uptake of the gastric wall was inhibited in both groups treated with acetyl-salicylic acid and bucolom, but it was not inhibited in either group treated with indomethacin and phenylbutazone.