Studies on alkaline phosphatase isozymes

Part 2. Alkaline phosphatase isozymes in the serum of various liver diseases

Abstract

Serum alkaline phosphatase isozymes were clearly separated by means of disc electrophoresis as described in part one of this paper, and might be useful for the differential diagnosis of liver diseases and the detection of original organ of the enzyme. Therefore, serum alkaline phosphatase in liver diseases was investigated by this method, and also, performed heat inactivation and L-phenylalanine inhibition in reference to original organs of alkaline phosphatase isozymes. The serum alkaline phosphatase isozymes in hepatoma and metastatic liver canser showed increased alkaline phosphatase isozyme at original point of the small pore Gel and liver alkaline phosphatase, and decreased bone alkaline phosphatase. This pattern of alkaline phosphatase isozymes was also seen in the early stage of liver cancer when other tests were negative. The intestinal alkaline phosphatase was seen in the most cases of liver cirrhosis and in a certain cases of hepatitis which indicated clinical value. Variant alkaline phosphatase of hepatoma, placental alkaline phosphatase and intestinal alkaline phosphatase were strongly inhibited by L-phenylalanine, and placental alkaline phosphatase is heatstable.