SCANNING ELECTRON MICROSCOPIC STUDIES ON THE EXPERIMENTAL ULCER OF THE RAT SMALL INTESTINE

I. Micro-angiological Observation on the Healing Process of the Small Intestinal Ulcer by the Injection Replica Scanning Electron Microscope Method

Abstract

Experimental ulcers were induced in the small intestine of rats by administration of hot water through a canulated tube. Healing process of ulcer was macroscopically divided into acute, healing and scarring stage. Micro-vascular changes were studied by injection replica scanning electron microscope method and injection Indian ink method. Following results were observed.
1) Acute stage: The villous capillary vessels were markedly dilated in the marginal zone, while vascular regeneration was rarely observed.
2) Healing stage: Various types of regenerating vessels were formed. Radial vessels were growing from serosa, and sprout like streight vessels were growing from submucosa. Regenerating capillary networks of villi and crypts were formed in the marginal zone of mucosa.
3) Scarring stage: The regenerating villous vessels were irregularly arranged and vascular networks were reduced.
4) Ulcer with prolonged healing: The regenerating vessels formed poor anastomosis, growing sparsely from serosa and submucosa.