長期アルコール飲用のラット肝フェノバルビタール誘導性チトクロームP-450量, 並びに小葉内分布に及ぼす影響

大西 久仁彦; 三島 昭彦; 波多野 等; 斉藤 正之; 中山 隆雅; 檜山 義明; 土屋 聖二; 野村 文夫; 岩間 章介; 河野 邦彦; 大槻 俊夫; 中嶋 征男; 石井 裕正; 奥田 邦雄

doi:10.11405/nisshoshi1964.80.1281

EFFECT OF CHRONIC ETHANOL FEEDING ON THE AMOUNT OF PHENOBARBITAL INDUCIBLE FORM OF CYTOCHROME P-450 AND ITS LOCALIZATION IN THE HEPATIC LOBULE OF THE RAT

Kunihiko OHNISHI, Akihiko MISHIMA, Hitoshi HATANO, Masayuki SAITO, Takatsune NAKAYAMA, Yoshiaki HIYAMA, Seiji TSUCHIYA, Fumio NOMURA, [in Japanese], Kunihiko KONO, Toshio OHTSUKI, Masao NAKAJIMA, Hiromasa ISHII, Kunio OKUDA

Author information

Kunihiko OHNISHI
First Department of Medicine, Chiba University Hospital
Akihiko MISHIMA
First Department of Medicine, Chiba University Hospital
Hitoshi HATANO
First Department of Medicine, Chiba University Hospital
Masayuki SAITO
First Department of Medicine, Chiba University Hospital
Takatsune NAKAYAMA
First Department of Medicine, Chiba University Hospital
Yoshiaki HIYAMA
First Department of Medicine, Chiba University Hospital
Seiji TSUCHIYA
First Department of Medicine, Chiba University Hospital
Fumio NOMURA
First Department of Medicine, Chiba University Hospital
[in Japanese]
First Department of Medicine, Chiba University Hospital
Kunihiko KONO
First Department of Medicine, Chiba University Hospital
Toshio OHTSUKI
First Department of Medicine, Chiba University Hospital
Masao NAKAJIMA
First Department of Medicine, Chiba University Hospital
Hiromasa ISHII
Department of Medicine, School of Medicine, Keio University
Kunio OKUDA
First Department of Medicine, Chiba University Hospital

JOURNAL FREE ACCESS

1983 Volume 80 Issue 6 Pages 1281-1287

DOI https://doi.org/10.11405/nisshoshi1964.80.1281

Details

Abstract

The form of cytochrome P-450 that is inducued by phenobarbital increased significantly after chronic ethanol feeding. It was identified by direct immunofluorescence in the hepatocyte cytoplasm throughout the hepatic lobule of the control rats, the ethanol-fed, and the phenobarbital-treated rats.
In the control rats, the fluorescence was barely disecenible but slightly more intense in the centrilobular than perilobular zones. In the ethanol-fed rats, the fluorescence was much more intense in the centrilobular zones, but was not as intense as that in the phenobarbital-treated rats. The tissue distribution and induction site of this component of the mixed function oxidases may be relevant to the site of an increased drug toxicity after chronic ethanol feeding as well as after phenobarbital treatment.

Corresponding author

Correction information

Register with J-STAGE for free!