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Tetsuo ARAKAWA, Hajime NAKAMURA, Shinji CHONO, Hiroaki YAMADA, Hiroshi ...
1983 Volume 80 Issue 6 Pages
1269-1271
Published: 1983
Released on J-STAGE: December 26, 2007
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We investigated the change in the proportion of several kinds of gastric epitherial cells by a longterm administration of 16, 16-dimethyl prostaglandin E
2 in rats. The increase in the number of mucus secreting cells and the decrease in the number of parietal cells were observed in rats received 16, 16-dimethyl prostaglandin E
2 for 2 and 10 weeks, suggesting that a long-term administration of the compound exerts a gastric protective effect through altering the proportion of gastric epithelial cells.
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Yuichi YAMASHITA, Michio MIYATA, Noboru YANAIHARA
1983 Volume 80 Issue 6 Pages
1272-1280
Published: 1983
Released on J-STAGE: December 26, 2007
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The intravenous administration of synthetic porcine GRP (pGRP) in the dog caused significant decrease of blood flow rates in celiac and superior mesenteric arteries with a transient increase in femoral artery. Atropine did not affect the stimulant action of pGRP on these arterial blood flow. The decrease of the superior mesenteric arterial blood flow by pGRP was dose-dependent both in the maximal reduction rate and the integrated reduction rate at the doses between 3.75 and 480pmol/kg i.v. bolus. Comparing the action of systemically synthesized pGRP and its related peptides upon the canine superior mesenteric arterial blood flow, one could conclude that the C-terminal octapeptide of pGRP was the vital active core of pGRP. The N-terminal tridecapeptide of pGRP was inactive upon the blood flow of superior mesenteric artery, but it seemed to be important for the full activity of pGRP.
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Kunihiko OHNISHI, Akihiko MISHIMA, Hitoshi HATANO, Masayuki SAITO, Tak ...
1983 Volume 80 Issue 6 Pages
1281-1287
Published: 1983
Released on J-STAGE: December 26, 2007
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The form of cytochrome P-450 that is inducued by phenobarbital increased significantly after chronic ethanol feeding. It was identified by direct immunofluorescence in the hepatocyte cytoplasm throughout the hepatic lobule of the control rats, the ethanol-fed, and the phenobarbital-treated rats.
In the control rats, the fluorescence was barely disecenible but slightly more intense in the centrilobular than perilobular zones. In the ethanol-fed rats, the fluorescence was much more intense in the centrilobular zones, but was not as intense as that in the phenobarbital-treated rats. The tissue distribution and induction site of this component of the mixed function oxidases may be relevant to the site of an increased drug toxicity after chronic ethanol feeding as well as after phenobarbital treatment.
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Shinichi OKADA
1983 Volume 80 Issue 6 Pages
1288-1298
Published: 1983
Released on J-STAGE: December 26, 2007
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The relationship between the amino acid composition of the culture media or pancreatic hormone and the nitrogen metabolism in primary monolayer cultured adult rat hepatocytes was studied. The composition of the amino acids used in the incubation media was similar to that of the plasma amino acids found in normal human subjects (NAA) as well as in patients with liver cirrhosis (LCAA). Synthesis rates of albumin and total protein were determined from the rates of
14C-leucine incorporation into immunoprecipitable albumin and into trichloroacetic acid-insoluble material. The results obtained were as follow:
1) Intact hepatocytes, as well as D-galactosamine-pretreated hepatocytes, when incubated in NAA, synthesized 1.1 to 1.2 times as much protein as hepatocytes incubated in LCAA. In protein synthesis, therefore, NAA was proved to have an advantage over LCAA.
2) Intact hepatocytes incubated in the media containing a physiological concentration of insulin (10
-9M), glucagon (3×10
-11M) and dexamethasone (10
-8M) synthesized about 1.5 times as much protein as hepatocytes cultured in the media without these hormones.
3) Glucagon at ten times the concentration of the physiological level stimulated protein synthesis in intact hepatocytes, particularly in those hepatocytes cultured in LCAA. The active amino acid transport mechanism may be stimulated by glucagon. These results suggest that hyperglucagonemia in cirrhotic patients may be a physiological response to plasma amino acid imbalance.
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Measurement of basal flow and its changes during food intake
Tamotsu KAWANO, Kazutomo INOUE, Koichi SHIMA, Shuhei HASHIDA, Takashi ...
1983 Volume 80 Issue 6 Pages
1299-1308
Published: 1983
Released on J-STAGE: December 26, 2007
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The dynamics of canine pancreatic blood flow were observed in the conscious state. Basal pancreatic blood flow under fasting and non-stimulus states was measured by the hydrogen gas clearance method. Pancreatic blood flow changes during meat meal ingestion were observed by the heated thermocouple method. Measurement in both methods was done by means of a tiny atraumatic element inserted into the pancreas.
The basal pancreatic blood flow was 50.7±17.3ml/min/100g in the conscious state. Various factors influencing basal pancreatic blood flow were analyzed by multivariate analysis, which showed that a intravenous pentobarbital injection (25mg/kg) caused a significant depression of the blood flow.
A pancreatic blood flow increment (maximum, 65%) immediately occured following meat meal ingestion. This increment returned slowly to the basal line over a period of 1-2 hours. In about half the animals, this response was biphasic, suggesting that the pancreatic blood flow response after food intake was composed of multiple factors.
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With special reference to γ-carboxyglutamic acid-containing protein
Akio YAMAGUCHI
1983 Volume 80 Issue 6 Pages
1309-1317
Published: 1983
Released on J-STAGE: December 26, 2007
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In order to elucidate the mechanism of formation of calculi in the pancrearic duct which are often found in the course of chronic pancreatitis, human pancreatic calculi were analyzed biochemically with attention focused on the affinity of γ-carboxyglutamic acid-containing protein for calciumions. The following results were obtained.
1. The main component of human pancreatic calculi was calcium carbonate.
2. Human calculi contained about 5% organic compounds.
3. Human pancreatic calculi contained a high concentration of protein (estimated molecular weight 14, 500) rich in acidic amino acids.
4. The presence of γ-carboxyglutamic acid as a constituent amino acid of this protein was confirmed.
Since γ-carboxyglutamic acid-containing protein had a specific affinity for calcium ions, γ-carboxyglutamic containing protein with an estimated molecular weight of 14, 500 was assumed to play an important role in the formation of pancreatic calculi composed mainly of calcium.
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Toshihisa TAKAHASHI, Shunji HASEGAWA, Shuji KAJIWARA, Yoshikura HARAGU ...
1983 Volume 80 Issue 6 Pages
1318-1326
Published: 1983
Released on J-STAGE: December 26, 2007
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We encountered 31 cases of pancreatic cysts in the past 13 years. They were calssified and clinically analysed and the literature reviewed. The cysts consisted of 13 pseudocysts, 15 retentions cysts, 2 cystadenomas and 1 cystadenocarcinoma. The pseudocysts originated from pancreatitis in 11 patients and from trauma in 2 patients. Abdominal pain was experienced by all the patients except two patients of 1 cystadenoma and 1 cystadenocarcinoma. Many patients presented with abdominal mass and elevated serum amylase. For difinitive diagnosis, we used to use intraoperative pancreatography but recently the diagnosis is made by such imaging modalities as ERP, CT-scanning and ultrasonography. Thirty patients received surgery; nine patients had direct operation on the cysts and 13 received pancreatic jejunostormy. Good results were obtained in these patients similarly to those who received cystectomy.
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Yutaka FUJITOMI, Yuzo UCHIDA, Okihiko SHIBATA, Kei BABA, Osamu SHIGEMI ...
1983 Volume 80 Issue 6 Pages
1327-1330
Published: 1983
Released on J-STAGE: December 26, 2007
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Masahiro SENJU, Muneharu KOMORI, Takashi IDE, Tunetoshi ICHIMURA, Masu ...
1983 Volume 80 Issue 6 Pages
1331-1335
Published: 1983
Released on J-STAGE: December 26, 2007
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Hirofumi SATO, Michihisa KOJIMA, Toshiki TATSUMURA, Keiichi YAMAMOTO, ...
1983 Volume 80 Issue 6 Pages
1336-1339
Published: 1983
Released on J-STAGE: December 26, 2007
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Takafumi BANDOH, Hiroshi TOYOSHIMA
1983 Volume 80 Issue 6 Pages
1340-1344
Published: 1983
Released on J-STAGE: December 26, 2007
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Report of a case with renal biopsy
Kiyoshi KAWAI, Eiichi TOMITA, Junichi SUGIHARA, Masami OHYAMA, Hiroo O ...
1983 Volume 80 Issue 6 Pages
1345-1348
Published: 1983
Released on J-STAGE: December 26, 2007
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Ching-Ming CHANG, [in Japanese], [in Japanese], [in Japanese], [in Jap ...
1983 Volume 80 Issue 6 Pages
1349
Published: 1983
Released on J-STAGE: December 26, 2007
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Isao MAKINO, [in Japanese], [in Japanese], [in Japanese], [in Japanese ...
1983 Volume 80 Issue 6 Pages
1350
Published: 1983
Released on J-STAGE: December 26, 2007
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Isao MAKINO, [in Japanese], [in Japanese], [in Japanese], [in Japanese ...
1983 Volume 80 Issue 6 Pages
1351
Published: 1983
Released on J-STAGE: December 26, 2007
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Takeo YAMANAKA, [in Japanese], [in Japanese], [in Japanese], [in Japan ...
1983 Volume 80 Issue 6 Pages
1352
Published: 1983
Released on J-STAGE: December 26, 2007
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