Rutin (0.3g.) was isolated from fresh flowers of Nicotiana Tabacum L., “Bright Yellow”, (3600g., with moisture). It was identified by absorption spectrographic and paper chromatographic methods (Tables 2, 3) and mp. 185-190°. The acetate of its aglucone melted at 193°, showing no melting point depression when melted simultaneously with, quercetin pentaacetate(mp., 192-193°).
