Abstract
We have studied quercetin, isoquercitrin (quercetin-3-glycoside from leaves of mulberry), quercitrin (quercetin-3-rhamnoside) and rutin (quercetin-3-rhamno-glycoside from buckweat) by paper partition chromatography.
The Rf values of these flavonoids by use of the upper layer of the three components system (50% ethyl acetate, 2% glacial acetic acid, and 50% distilled water) were as follows: quercetin 0.80, quercitrin 0.67, isoquercitrin 0.43 and rutin 0.10.
The spectar of these flavonoids extracted from the spot were identical with the spectra of original flavonoids.
These glycosides hydolysed wiht dil. H2SO4, gave the quercetin. glucose and rhamnose spots by paper chromatography.
Isoquercitrin was detected on chromatogram by partial decomposition of rutin in alcoholic solution.
By this method, it, is possible to separate and determine these flavonoids and study its chemical structure.
