Abstract
The end group assay was carried out with periodate oxidation method on several starchtype polysaccharides, and average number of glucose residue per non-reducing end group was calculated from the amount of formic acid liberated after 25 hours of oxidation by 0.037 M sodium metaperiodate at 0° The following values of average chain length were obtaind: soluble potato starch 29.5, potato amylopectin 26.3, potato amylose 480, soluble glutinous-ricestarch 18, and polysaccharide produced from glutinousrice starch by the action of isoamy lase 100±5. The value for the product of isoamylase approximately coincided with values of average polymerisation Ldegree, formerly estimated with viscosimetryor osmometry.
From the above result the authors have confirmed KOBAYASHI's theory that the isoamylase produces amylose-type polysaccharide from amylopectin-type polysaccharide as previously, reported(1)(2).
