Anti-Rennin Factor in Swine Serum Part IV

Anti-Rennin Substance and its Function

Abstract

(1) By fractionation, an anti-rennin factor (ARF) was isolated from swine blood serum. It was a salt containing Cl, Na, Ca, and Mg. (2) ARF was crystallized easily from its water solution. The crystalline ARF became white powder when it was desiccated. The powdered ARF contained about 92% of ash. The ash formed by incineration of ARF lost a part of the anti-rennin activity. (3) When a solution from ARF and serum protein, adjusted its pH to that of blood, was added to milk, more anti-rennin activity was observed, compared with the case when ARF alone was used in the clotting test of the milk. (4) ARF prevented milk not only from clotting by rennin but also from clotting by pepsin.