Abstract
L-Phe was precipitated by forming adduct with benzoic acid and its derivatives, for example, m- and p-fluoro, m-chloro, m-bromo, p-hydroxy, m- and p-nitrobenzoic acid and etc. Their o-isomers generally failed to form adduct. An adduct of benzoic acid was isolated and ratio of Phe to benzoic acid was equimolar. Optimal pH for the adduct formation was 3_??_4 and the adduct formation failed at pH 1.0 L-Phe was selectively separated from L-Leu and L-Ile mixture as the adduct of benzoic acid. L-Leu and L-Ile formed no adduct with benzoic acid.
