Abstract
The browning reaction solution, obtained by heating an aqueous solution (pH over 9) of ammonia (3M) and D-glucose (1M) at 100°C for 7 hr, was dialyzed, and both outer and inner solution were fractionated by ion-exchange, thin layer, and gel-permeation chro-matographies. The outer solution was passed through a column of DEAE-cellulose and the water-eluted fraction was separated by preparative thin layer chromatography with cellulose powder into four clear bands. The main band was much effective in inhibiting autoxidation of purified linolenic acid solution. The antioxidant activity, however, was distributed all over the colored fractions; comparing at the same optical density (490mμ), lower molecular weight fractions were stronger than higher molecular weight fractions. The antioxidant activity of the browning reaction products was found to be hardly dependent on the hydrogen- or electron-donating activity which was estimated by the reaction with 2, 2'-diphenyl-1-picrylhydrazyl.
