Abstract
32P-Ciliatine was injected intraperitoneally into growing Wistar rats. After the injection, 32P-ciliatine was incorporated into almost all rat tissues. Incorporation of 32P-ciliatine into the liver amounted to ca. 17.5% of the dose, while its incorporation into the other tissues was much lowered, e. g., kidney, 1.23%; small intestine, 0.20%; skeletal (hind leg) muscle, 0.07%; bone (hind leg bone), 0.06%. From the values of C-32P/T-32P (%) in all tissues, radioactivity was detected mostly as ciliatine, but a small portion was found in some changed form. This observation suggests that ciliatine can be decomposed in the rat tissues or by gastrointestinal bacteria.
Further studies on the incorporation of 32P-ciliatine into the rat liver at each of nine intervals (0.5, 1, 2, 4, 8, 16, 32, 64, 128 hr) after the intraperitoneal injection indicated that ciliatine incorporation reached maximum after 4 hr, then decreased slowly, and that ciliatine is firstly incorporated into the acid soluble fraction of rat liver and then into the other fractions, especially into the phospholipid fraction.
Male rats weighing about 50g were fed three kinds of diet: a low-phosphorus, a low-phosphorus+ciliatine and a control diet. Ciliatine administered orally was considerably accumulated in the liver and many other tissues. In the liver which largely accumulated, the phospholipid fraction showed much higher than any other fractions. Ciliatine, even if administered orally in large quantity, has no apparently toxic effect on growing rats, but partly any substitutional effect for common phosphorus compounds on their growth.
