Abstract
It was found that there are two types of saccharases developed in the aged slices of sugar beet root. One of them was soluble enzyme and another was insoluble saccharase bound to the cell wall. The hydrolysis of sucrose was largely due to the actinity of the bound type-saccharase. By treating the cell wall with NaCl, KCl, NaNO3, KNO3 or Na2SO4 solutions, the bound enzyme was liberated and subsequently solubilized. The concentra-tion of NaCl or KCl more than 0.3M and of MgSO4 more than 0.1M were required to solubilize the enzyme.
The maximum activity of the bound saccharase was found to be at pH 4.6 and was stable at pH values from 4.0 to 7.0. The enzyme was inactivated by the preincubation at 45°C for 15min. The Km value was determined to be 1.53mM for sucrose. The en-zyme was considerably inhibited by HgCl2, FeSO4 and PCMB.
