Abstract
The enzymic reaction on sucrose was carried out with the invertase of pollen sterilized by γ-rays. From the reaction mixture three oligosaccharides were isolated, one of which was found to be 1-kestose.
Pollen invertase was a β-fructofuranosidase having trans-D-fructosylase activity, while honeybees invertase was an α-glucosidase.
1-Kestose is formed presumably by trans-D-fructosylation catalyzed with pollen invertase.
