Abstract
In general, the ion exchange resin process has been industrially applied to isolate amino acids from their fermented broth. The theoretical expressions for the equilibrium of amino acids on ion exchange resin are already reported. However, they can not be applied to the amino acids isolation from their fermented broth, because the fermented broth includes too many kinds of organic and inorganic components to determine the constants of theoretical expressions. Therefore, we tried to develop the practical expression for the ion exchange equilibrium in terms of the application of the law of mass action in the case of the isolation of lysine from fermented broth. The two types of lysine in the acid division, i.e. monovalent cationic lysine and bivalent cationic lysine, should be considered as well as the other inorganic ions.
We measured the relative affinities especially for these two types of lysine to the strong acid cation exchange resin (D. V. B. 8%). By means of these relative affinities in our expression, we calculated the theoretical capacities of adsorption for lysine and compared them with the experimental values found for the prepared lysine broth. From these data our expressions and the values of relative affinities were found to be roughly applicable to know the capacities and the optimun conditions for the adsorption of amino acids onto the strong acid cation exchange resin with their fermented broth.
