Partial Purification of Cyanide Metabolizing Activity in Mesocarp of Loquat
1977 Volume 51 Issue 9 Pages 527-530
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1977 Volume 51 Issue 9 Pages 527-530
Free cyanide contents in loquat endosperm and mesocarp were determined to be 203±13 ppm and 0.22±0.08 ppm respectively.
The cyanide metabolizing activity was partially purified about 430-fold by ammonium sulfate precipitation, Sephadex G-100 gel filtration and isoelectric focusing. The optimum pH for this activity was 8.0. Stoichiometric NH3 liberation from CN- was observed using the partially purified fraction.
