Isolation of Dehydroacetic Acid Degradating Bacteria and Metabolic Products from Dehydroacetic Acid

Abstract

Four kinds of bacteria, which metabolized dehydroacetic acid, were isolated from mid-air and soil using enrichment cultivation containing dehydroacetic acid in the medium as a sole source of carbon. Among those bacteria, two kinds of bacteria, C-5-1 from mid-air and M-1 from soil, were designated as Pseudomonas sp. C-5-1 and Pseudomonas sp. M-1, respectively. It was found that degradation of dehydroacetic acid by Pseudomonas sp. C-5-1 was more rapid than that of Pseudomonas sp. M-1.
The metabolic pathway of dehydroacetic acid by both strains was assumed as follows: dehydroacetic acid→triacetic acid lactone→triacetic acid→acetic acid→TCA cycle. Furthermore, the accumulation of triacetic acid by Pseudomonas sp. M-1 was strongly affected by the presence of ammonium nitrate in culture medium, but with Pseudomonas sp. C-5-1 such an effect was not observed.