Abstract
HmLu-1 cells established from humster lung tissue was found to grow in a serum-free medium indefinitely. This enabled to simplify the purification of bovine virus particles multiplied in the cells and come into the medium, since no serum proteins came to contaminate in the virus preparation. Partially purified virus particles were injected to BALB/c mice intraperitoneally to sensitize B-lymphocytes producing antibodies specific to the virus antigen. Spleen cells of the mice were then fused with a hypoxanthine-aminopterin-thymidine (HAT) sensitive mouse myeloma cell line, P3X63Ag8U1, using polyethyleneglycol. Hybridoma lines secreting monoclonal antibodies specific to each viruses were obtained and cloned. The cross reactivities of these monoclonal antibodies against akabane, ephemeral fever, ibaraki and chuzan viruses were examined. The combined use of these virus-specific monoclonal antibodies will facilitate rapid detection of virus-infected tissues and individuals.
